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KMID : 0043320150380101913
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Archives of Pharmacal Research
2015 Volume.38 No. 10 p.1913 ~ p.1920
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Inhibitory effect of acteoside on melittin-induced catecholamine exocytosis through inhibition of Ca2+-dependent phospholipase A2 and extracellular Ca2+ influx in PC12 cells
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Song Ho-Sun
Ko Myung-Soo
Jo Young-Soo
Whang Wan-Kyunn
Sim Sang-Soo
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Abstract
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To investigate the inhibitory effect of acteoside on the process of exocytosis induced by melittin, we measured Ca2+ mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca2+ mobilization via receptor-operated calcium channel but not the intracellular Ca2+ release. It caused AA release via activation of Ca2+-dependent phospholipase A2 (PLA2) and catecholamine secretion in a dose-dependent manner. Acteoside dose-dependently inhibited the release of AA and intracellular Ca2+ mobilization induced by melittin. Acteoside reduced the catecholamine release and raised the amount of intracellular chromogranin A which is co-released with catecholamine from melittin-stimulated PC12 cells. Taken together, our results suggest that acteoside could suppress the exocytosis via inhibition of Ca2+-dependent PLA2 and extracellular Ca2+ influx in PC12 cells stimulated by melittin.
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KEYWORD
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Acteoside, Arachidonic acid, Catecholamine, Ca2+ mobilization, Chromogranin A, Phospholipase A2
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